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Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients
Noronha, Samuel Marcos Ribeiro de; Noronha, Silvana Aparecida Alves Corrêa de; Klepp, Anthony Gueratto; Ipolito, Michele Zampieri; Ferreira, Lydia Masako; Gragnani, Alfredo.
  • Noronha, Samuel Marcos Ribeiro de; UNIFESP. EPM. Department of Surgery. Sao Paulo. BR
  • Noronha, Silvana Aparecida Alves Corrêa de; UNIFESP. EPM. Department of Surgery. Sao Paulo. BR
  • Klepp, Anthony Gueratto; UNIFESP. EPM. Department of Surgery. Sao Paulo. BR
  • Ipolito, Michele Zampieri; UNIFESP. EPM. Department of Surgery. Sao Paulo. BR
  • Ferreira, Lydia Masako; UNIFESP. EPM. Department of Surgery. Sao Paulo. BR
  • Gragnani, Alfredo; UNIFESP. EPM. Department of Surgery. Sao Paulo. BR
Acta cir. bras ; 29(supl.1): 62-68, 2014. tab, graf
Article in English | LILACS | ID: lil-720401
ABSTRACT

PURPOSE:

To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn.

METHODS:

Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supernatant of the culture medium was measured by, respectively, flow cytometry using Cytometric Bead Array Human Inflammation kit (CBA, BD Biosciences, USA) and the enzyme immunoassay method using the Quantikine (r) Human KGF. The experiments were performed in triplicate.

RESULTS:

The expression of IL-12 protein in patients with large burns showed a tendency to increase. IL- 6, IL- 10, and IL- 1beta were observed no difference. For IL - 8, TNF - alpha and KGF was observed a significant difference between the expression in large and small burned patient.

CONCLUSION:

That IL-8, TNF-alpha and KGF showed higher expression in cultured fibroblasts of large burned patients. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Skin / Burns / Interleukins / Tumor Necrosis Factor-alpha / Culture Media / Fibroblasts Limits: Adult / Female / Humans / Male Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2014 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR

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Full text: Available Index: LILACS (Americas) Main subject: Skin / Burns / Interleukins / Tumor Necrosis Factor-alpha / Culture Media / Fibroblasts Limits: Adult / Female / Humans / Male Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2014 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: UNIFESP/BR