Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis
Acta cir. bras
;
29(9): 544-552, 09/2014. graf
Article
in English
| LILACS
| ID: lil-722131
ABSTRACT
PURPOSE:
To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.METHODS:
Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05).RESULTS:
Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention.CONCLUSIONS:
Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream. .
Full text:
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Index:
LILACS (Americas)
Main subject:
Sucralfate
/
Membrane Glycoproteins
/
Colitis
/
Enema
/
Anti-Ulcer Agents
/
Mucins
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Sao Francisco University/BR
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