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Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil
Silva, Rodrigo Otávio Silveira; Vilela, Eduardo Garcia; Neves, Monique Silva; Lobato, Francisco Carlos Faria.
  • Silva, Rodrigo Otávio Silveira; Universidade Federal de Minas Gerais. Faculdade de Medicina Veterinária. Belo Horizonte. BR
  • Vilela, Eduardo Garcia; Universidade Federal de Minas Gerais. Faculdade de Medicina Veterinária. Belo Horizonte. BR
  • Neves, Monique Silva; Universidade Federal de Minas Gerais. Faculdade de Medicina Veterinária. Belo Horizonte. BR
  • Lobato, Francisco Carlos Faria; Universidade Federal de Minas Gerais. Faculdade de Medicina Veterinária. Belo Horizonte. BR
Rev. Soc. Bras. Med. Trop ; 47(4): 447-450, Jul-Aug/2014. tab
Article in English | LILACS | ID: lil-722303
ABSTRACT
Introduction Despite the known importance of Clostridium difficile as a nosocomial pathogen, few studies regarding Clostridium difficile infection (CDI) in Brazil have been conducted. To date, the diagnostic tests that are available on the Brazilian market for the diagnosis of CDI have not been evaluated. The aim of this study was to compare the performances of four commercial methods for the diagnosis of CDI in patients from a university hospital in Brazil. Methods Three enzyme immunoassays (EIAs) and one nucleic acid amplification test (NAAT) were evaluated against a cytotoxicity assay (CTA) and toxigenic culture (TC). Stool samples from 92 patients with suspected CDI were used in this study. Results Twenty-five (27.2%) of 92 samples were positive according to the CTA, and 23 (25%) were positive according to the TC. All EIAs and the NAAT test demonstrated sensitivities between 59 and 68% and specificities greater than 91%. Conclusions All four methods exhibited low sensitivities for the diagnosis of CDI, which could lead to a large number of false-negative results, an increased risk of cross-infection to other patients, and overtreatment with empirical antibiotics. .
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Full text: Available Index: LILACS (Americas) Main subject: Clostridioides difficile / Immunoenzyme Techniques / Clostridium Infections / Nucleic Acid Amplification Techniques / Diarrhea Type of study: Diagnostic study / Evaluation studies / Risk factors Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR

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Full text: Available Index: LILACS (Americas) Main subject: Clostridioides difficile / Immunoenzyme Techniques / Clostridium Infections / Nucleic Acid Amplification Techniques / Diarrhea Type of study: Diagnostic study / Evaluation studies / Risk factors Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR