Actividad antioxidante y antihiperglucemiante de la especie medicinal Oreocallis grandiflora (Lam.) R. Br., al sur del Ecuador / Antioxidant and antihyperglycemic activity of the medicinal specie Oreocallis grandiflora (Lam.) R. Br., in southern Ecuador
Bol. latinoam. Caribe plantas med. aromát
;
12(1): 59-68, ene. 2013. tab
Article
in Spanish
| LILACS
| ID: lil-722508
ABSTRACT
In this study we evaluated the antioxidant and antihyperglycemic activity in vitro of the extracts obtained with solvents hexane, ethyl acetate and methanol, of the medicinal plant Oreocallis grandiflora (cucharillo), collected in the Saraguro indian community of the province Loja, southern Ecuador. The antioxidant activity was evaluated by the tests DPPH, FOLIN-CIOCALTEU and beta-CLAMS, while the antihyperglycemic activity was determined by inhibition assay á-amylase and alpha-glucosidase. The samples were diluted to different concentrations and the reading was performed in a UV spectrophotometer, using as positive control á-tocopherol for DPPH and Folin-ciocalteu test, trolox for beta-CLAMS test, and Glucobay® for testing alpha-amylase and alpha-glucosidase.The results are expressed as IC50, these show that the methanol extract of Oreocallis grandiflora has inhibitory effect on alpha-amylase, the IC50 is 109 ug/ml, compared to 126 ug/ ml of Glucobay®. It also shows inhibitory effect on á-glucosidase, the IC50 is 3 ug/ml compared to 1316 ug/ml of Glucobay®. It also shows antioxidant activity, its IC50 is 15 ug/ml compared to 5 ug/ml of á-tocopherol.
RESUMEN
En el presente trabajo se evaluó la actividad antioxidante y antihiperglucemiante in vitro de los extractos obtenidos con los solventes hexano, acetato de etilo y metanol, de la planta medicinal Oreocallis grandiflora (cucharillo), recolectada en la comunidad indígena de Saraguro en la provincia de Loja, al sur del Ecuador. La actividad antioxidante fue evaluada a través de los ensayos DPPH, FOLIN-CIOCALTEU y beta-CLAMS, mientras que la actividad antihiperglucemiante fue determinada por el ensayo de inhibición de alfa- amilasa y alfa-glucosidasa. El extracto metanólico de Oreocallis grandiflora presenta efecto inhibitorio sobre la enzima alfa-amilasa, su concentración inhibitoria (CI50) es de 109 ug/ml, frente a 126 ug/ml del control positivo Glucobay®. Además, muestra efecto inhibitorio sobre la enzima alfa-glucosidasa, su concentración inhibitoria (CI50) es de 3 ug/ml, frente a 1316 ug/ml del Glucobay®. Muestra también actividad antioxidante, su concentración inhibitoria (CI50) es de 15 ug/ml, frente a 5 ug/ml del alfa-tocoferol.
Full text:
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Index:
LILACS (Americas)
Main subject:
Plant Extracts
/
Proteaceae
/
Hypoglycemic Agents
/
Antioxidants
Country/Region as subject:
South America
/
Ecuador
Language:
Spanish
Journal:
Bol. latinoam. Caribe plantas med. aromát
Journal subject:
Botany
/
Medicine
/
Plantas Medicinais
/
Terapias Complementares
Year:
2013
Type:
Article
Affiliation country:
Ecuador
Institution/Affiliation country:
Universidad Técnica Particular de Loja/EC
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