Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
Braz. j. microbiol
;
45(2): 389-393, Apr.-June 2014. ilus
Article
in English
| LILACS
| ID: lil-723093
ABSTRACT
Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have been made inaccessible by dimerisation, thus rendering the lipase resistant to protease.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacillus
/
Lipase
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2014
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Vels University/IN
Similar
MEDLINE
...
LILACS
LIS