Molecular detection of Brucella species in patients suspicious of Brucellosis from Zanjan, Iran
Braz. j. microbiol
;
45(2): 533-538, Apr.-June 2014. ilus, tab
Article
in English
| LILACS
| ID: lil-723114
ABSTRACT
Brucella is an intracellular pathogen capable of infecting animals and humans. The aim of this study was to identify Brucella spp in sera of high risk individuals by a polymerase chain reaction (PCR)-based method. A total of 180 patients suspected to have Brucellosis were examined by serological tests. To establish a PCR protocol for diagnosis of active brucellosis, DNA was extracted from the serum samples by using a commercial kit. PCR amplification was done for detection of Brocella DNA using BCSP31 target gene and IS711 locus. The PCR assay showed that an amplicon of 223 bp was obtained in 73.8% (133/180) of the tested sera using primers (B4/B5) derived from a gene encoding the 31-kDa Brucella abortus antigen. In another PCR, an amplicon of 498 bp was obtained in 63.8% (115/180) of the samples using Brucella abortus-specific primers derived from a locus adjacent to the 3'-end of IS711, and also an amplicon of 731 bp was produced in 4.4% (8/180) of the tested samples using Brucella melitensis-specific primers. When the Wright method was used as a gold standard, the sensitivity and specificity of the PCR technique for genus identification were found to be 96 and 80.7%, respectively. However, the sensitivity value obtained with the species-specific PCR method was 82%, and specificity was similar to that previous reported. This is the first report of a high frequency of Brucella abortus in patients suspicious of Brucellosis from the Zanjan province.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Brucella abortus
/
Brucellosis
/
Polymerase Chain Reaction
/
Brucella melitensis
/
Molecular Diagnostic Techniques
/
Serum
Type of study:
Diagnostic study
/
Evaluation studies
/
Practice guideline
/
Prognostic study
Limits:
Animals
/
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2014
Type:
Article
Affiliation country:
Iran
Institution/Affiliation country:
Islamic Azad University/IR
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