Search for endophytic diazotrophs in barley seeds

S. Zawoznik, Myriam; C. Vázquez, Susana; Díaz Herrera, Silvana M.; D. Groppa, María

S. Zawoznik, Myriam; C. Vázquez, Susana; Díaz Herrera, Silvana M.; D. Groppa, María.

S. Zawoznik, Myriam; Universidad de Buenos Aires. Departamento de Química Biológica. Buenos Aires. AR
C. Vázquez, Susana; Universidad de Buenos Aires. Departamento de Química Biológica. Buenos Aires. AR
Díaz Herrera, Silvana M.; Universidad de Buenos Aires. Departamento de Química Biológica. Buenos Aires. AR
D. Groppa, María; Universidad de Buenos Aires. Departamento de Química Biológica. Buenos Aires. AR

Braz. j. microbiol ; 45(2): 621-625, Apr.-June 2014. ilus, tab

Article in English | LILACS | ID: lil-723126

ABSTRACT

ABSTRACT

Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro and showed no antibiosis effects against Azospirillum brasilense Az39, a recognized PGPR.

Subject(s)

Azospirillum brasilense/isolation & purification; Bacillus/isolation & purification; Endophytes/isolation & purification; Hordeum/microbiology; Nitrogen Fixation; Pseudomonas/isolation & purification; Seeds/microbiology; Antibiosis; Azospirillum brasilense/classification; Azospirillum brasilense/genetics; Azospirillum brasilense/metabolism; Bacillus/classification; Bacillus/genetics; Bacillus/metabolism; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; DNA, Ribosomal/chemistry; DNA, Ribosomal/genetics; Endophytes/classification; Endophytes/genetics; Endophytes/metabolism; Indoleacetic Acids/metabolism; Molecular Typing; Pseudomonas/classification; Pseudomonas/genetics; Pseudomonas/metabolism; Random Amplified Polymorphic DNA Technique; /genetics; Sequence Analysis, DNA

Azospirillum; Bacillus; PGPR; Pseudomonas; RAPD

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Full text: Available Index: LILACS (Americas) Main subject: Pseudomonas / Seeds / Bacillus / Hordeum / Azospirillum brasilense / Endophytes / Nitrogen Fixation Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2014 Type: Article / Project document Affiliation country: Argentina Institution/Affiliation country: Universidad de Buenos Aires/AR

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