Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
J. venom. anim. toxins incl. trop. dis
;
2004/02/2014.
Article
in English
| LILACS
| ID: lil-724683
ABSTRACT
Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasites DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasites DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Leishmaniasis
/
Polymerase Chain Reaction
/
Diagnosis
/
False Negative Reactions
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Pesquisas Aggeu Magalhães/BR
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