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Establishment of a pheasant (Phasianus colchicus) spermatogonial stem cell line for the production of interspecies germ line chimeras
Kim, Jeong Hyun; Sharma, Neelesh; Kim, Sung Woo; Sodhi, Simrinder Singh; Ghosh, Mrinmoy; Kim, Nam Eun; Mongre, Raj Kumar; Oh, Sung Jong; Jeong, Dong Kee.
  • Kim, Jeong Hyun; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Sharma, Neelesh; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Kim, Sung Woo; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Sodhi, Simrinder Singh; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Ghosh, Mrinmoy; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Kim, Nam Eun; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Mongre, Raj Kumar; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Oh, Sung Jong; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
  • Jeong, Dong Kee; Jeju National University. Faculty of Biotechnology. Department of Animal Biotechnology. Jeju. KR
Electron. j. biotechnol ; 17(5): 211-216, Sept. 2014. ilus, tab
Article in English | LILACS | ID: lil-724786
ABSTRACT
Background Spermatogonial stem cells (SSCs) are important for the production of interspecies germ line chimeras. The interspecies germ cell transfer technique has been suggested as a way to conserve endangered birds. Our objective was to develop a technique for restoring endangered birds by developing interspecies germ line chimeras between pheasant (Phasianus colchicus) and chicken (Gallus gallus) with SSCs. Results SSCs were isolated from the surgically removed testis of a pheasant. Growth conditions for pheasant SSCs were established by co-culturing STO (SIM mouse embryo-derived thioguanine and ouabain resistant) cells and pheasant SSCs. The colony-forming cells divided and proliferated stably to yield an established SSC line. Pheasant SSCs showed strong reactivity for GDNF family receptor alpha1 (GFRa1) marker. Finally, production of germ line chimeras was attempted by transferring pheasant SSCs into recipient embryos. Although final embryo survival was 5.6% (20/354), the initial survival rate was 88% (312/354). To measure the percent transfer of donor SSC to gonads, the pheasant SSCs were labeled with PKH 26 fluorescent dye. We observed 30% donor cells and 9.48% c-kit/CD117-positive cells in the gonads of recipient chickens. Donor SSCs were thus stably engrafted in the recipient gonads. Conclusions This study showed that SSCs can be used as a tool for the conservation of endangered birds and the production of germ line chimeras. Our findings yield insights into how we may use the pheasant spermatogonial stem cell line for efficient production of interspecies germ line chimeras and ultimately, to the restoration of endangered birds.
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Full text: Available Index: LILACS (Americas) Main subject: Spermatogonia / Stem Cells / Stem Cell Transplantation / Galliformes Limits: Animals Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2014 Type: Article Affiliation country: South Korea Institution/Affiliation country: Jeju National University/KR

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Full text: Available Index: LILACS (Americas) Main subject: Spermatogonia / Stem Cells / Stem Cell Transplantation / Galliformes Limits: Animals Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2014 Type: Article Affiliation country: South Korea Institution/Affiliation country: Jeju National University/KR