Animal model of chronic kidney disease using a unilateral technique of renal ischemia and reperfusion in White New Zealand rabbits
Acta cir. bras
;
29(10): 651-657, 10/2014. tab, graf
Article
in English
| LILACS
| ID: lil-725293
ABSTRACT
PURPOSE:
To establish a model of chronic kidney disease in White New Zealand rabbits, using an exclusive unilateral technique of renal ischemia and reperfusion.METHODS:
Twenty males White New Zealand rabbits were used. All animals were subjected to the following pre-surgical blood collection (1st collection) for creatinine and urea serum analysis, left renal ischemia and reperfusion surgery technique, another blood sample was collected after 6 weeks post surgery (2nd collection), the last blood sample (3rd collection) blood sample was taken 11 weeks post surgery (pre-euthanasia), euthanasia and withdrawal of right and left kidney for histopathological analysis.RESULTS:
The creatinine levels after surgery was statistically significant higher in the 3rd collection, regarding the 1st and 2nd collection (p<0.05). A significant statistic increase for urea was showed only in the 2nd collection (p<0.05) when compared to the 1st and 3rd collections. Histopathological analysis showed bilateral lesions in the renal tissue, consistent to the process of ischemia and reperfusion.CONCLUSION:
This exclusive unilateral technique of renal ischemia and reperfusion without nephrectomy in White New Zealand rabbits, showed effectiveness in getting an animal model of chronic kidney disease .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Reperfusion Injury
/
Disease Models, Animal
/
Renal Insufficiency, Chronic
/
Kidney
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Mato Grosso do Sul/BR
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