Effectiveness of high-power diode laser associated to chemical auxiliary substance on decontamination of root canal system infected with Enterococcus faecalis ? an in vitro study
RFO UPF
;
18(3): 288-294, set.-dez. 2013.
Article
in English
|
LILACS-Express
| LILACS
| ID: lil-726475
ABSTRACT
Objective:
to evaluate the additional antibacterial ac-tion of high-power diode laser after the use of 2.5% sodium hypochlorite and 2% chlorhexidine gel in root canals infected with E.faecalis. Materials andmethod:
120 human teeth with single canal were inoculated with E.faecalis for 14 days and randomly divided into six groups (n=20), according to the chemical auxiliary substance used in the chemo-mechanical preparation and the use of high-power diode laser G1(NaOCl) ? root canal preparation with 2.5% sodium hypo-chlorite; G2(CHX) ? root canal preparation with 2% chlorhexidine gel; G3(NaOCl+DL) - root canal pre-paration with 2.5% sodium hypochlorite+diode la-ser; G4(CHX+DL) - root canal preparation with 2% chlorhexidine gel+diode laser; G5(NaCl+DL) - root canal preparation with 0.9% saline solution+diode la-ser; G6(no treatment) ? no treatment was performed. Microbiological test (CFUs counting) was performed to evaluate the effectiveness of proposed treatments. Data were subjected to One-way ANOVA followed by post-hoc Tukey test (?=0.05).Results:
G3(NaOCl+DL) had reduction of 99.42% of E.faecalis, followed by G4(CHX+DL) ? 93.48%; G1(NaOCl) ? 89.82%; G2(CHX) ? 81.86%; G5(NaCl+DL) ? 65.40%; and G6(no treatment) ? no reduction. There was significant statistical difference in CFUs of all groups in relation to G6(no treatment) (p<0.05). G1(NaOCl), G2(CHX), G3(NaOCl+DL) and G4(CHX+DL) were statistically superior to G5(NaCl+DL) (p<0,05).Conclusion:
High--power diode laser, when associated with 2.5% NaOCl or 2% CHX, improves the antimicrobial effect of these substances in the root canals infected with E. faecalis.
Full text:
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Index:
LILACS (Americas)
Type of study:
Risk factors
Language:
English
Journal:
RFO UPF
Journal subject:
Dentistry
Year:
2013
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
MSc, Department of Restorative Dentistry, School of Dentistry, University of Passo Fundo/BR
/
PhD School of Agriculture and Veterinary Medicine, University of Passo Fundo/BR
/
PhD, Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul/BR
/
PhD, Department of Restorative Dentistry, School of Dentistry, University of Passo Fundo/BR
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