Caracterización de línea celular de paratiroides humana (RCPTH) y evaluación de tumorigenicidad en ratones NOD/SCID / Characterization of human parathyroid cell line (RCPTH) and tumorigenicity evaluation in NOD/SCID
Rev. chil. endocrinol. diabetes
;
6(2): 59-68, abr. 2013. ilus, tab, graf
Article
in Spanish
| LILACS
| ID: lil-726576
ABSTRACT
Background:
Cell therapy could be an alternative for the treatment of hypoparathyroidism. Therefore efforts have been made to establish a cell line of parathyroid cells.Aim:
To establish a continuous functional and non-tumorigenic human parathyroid cell line. Material andMethods:
Nineteen tissue samples from 15 patients subjected to parathyroidectomy due to primary or secondary hyperparathyroidism were obtained. Functional, morphological and tumorigenic properties of the obtained cells were analyzed.Results:
After two months of culture in conditions of immortalization, cells had an exponential growth without experiencing senescence. Therefore, more than 200 sub cultures have been performed. The cell line was denominated RCPTH. Morphological characterization showed monolayer growth with contact inhibition and a duplication time of 30 hours. On light microscopy, pleomorphism and low number of mitoses were observed. Cells accumulated glycogen, expressed calcium sensing receptor and had positive PTH cytoplasmic clusters. The line secreted PTH initially but subsequently, PTH production became undetectable. The cell line did not have tumor or metastatic growth.Conclusions:
A parathyroid cell line has been established. The lack of PTH production is a problem that will require the search for mechanisms to activate it.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Parathyroid Glands
/
Cell Transformation, Neoplastic
/
Cell Transplantation
Limits:
Animals
/
Humans
Language:
Spanish
Journal:
Rev. chil. endocrinol. diabetes
Journal subject:
Endocrinology
Year:
2013
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Hospital Clínico Universidad de Chile/CL
Similar
MEDLINE
...
LILACS
LIS