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Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures
Pinheiro, Luiza; Brito, Carla Ivo; Pereira, Valéria Cataneli; Oliveira, Adilson de; Camargo, Carlos Henrique; Cunha, Maria de Lourdes Ribeiro de Souza da.
  • Pinheiro, Luiza; Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Departamento de Microbiologia e Imunologia. Laboratório de Microbiologia. Botucatu. BR
  • Brito, Carla Ivo; Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Departamento de Microbiologia e Imunologia. Laboratório de Microbiologia. Botucatu. BR
  • Pereira, Valéria Cataneli; Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Departamento de Microbiologia e Imunologia. Laboratório de Microbiologia. Botucatu. BR
  • Oliveira, Adilson de; Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Departamento de Microbiologia e Imunologia. Laboratório de Microbiologia. Botucatu. BR
  • Camargo, Carlos Henrique; Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Departamento de Microbiologia e Imunologia. Laboratório de Microbiologia. Botucatu. BR
  • Cunha, Maria de Lourdes Ribeiro de Souza da; Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Departamento de Microbiologia e Imunologia. Laboratório de Microbiologia. Botucatu. BR
Mem. Inst. Oswaldo Cruz ; 109(7): 871-878, 11/2014. tab
Article in English | LILACS | ID: lil-728793
ABSTRACT
This study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (MIC) was measured for isolates and subpopulations growing on vancomycin screen agar. The mecA gene was detected in 81.3% of the S. epidermidis isolated and 48.2% carried SCCmec type III. The complete icaADBC operon was observed in 38.3% of the isolates; of these, 58.5% produced a biofilm. Furthermore, 47.7% of the isolates grew on vancomycin screen agar, with an increase in the MIC in 75.9% of the isolates. Determination of the MIC of subpopulations revealed that 64.7% had an MIC ≥ 4 μg mL-1, including 15.7% with an MIC of 8 μg mL-1 and 2% with an MIC of 16 μg mL-1. The presence of the icaADBC operon, biofilm production and reduced susceptibility to vancomycin were associated with methicillin resistance. This study reveals a high level of methicillin resistance, biofilm formation and reduced susceptibility to vancomycin in subpopulations of S. epidermidis. These findings may explain the selection of multidrug-resistant isolates in hospital settings and the consequent failure of antimicrobial treatment.
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Full text: Available Index: LILACS (Americas) Main subject: Operon / Staphylococcal Infections / Staphylococcus epidermidis / Methicillin Resistance / Biofilms / Vancomycin Resistance Limits: Adult / Aged / Female / Humans / Infant / Male / Infant, Newborn Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2014 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR

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Full text: Available Index: LILACS (Americas) Main subject: Operon / Staphylococcal Infections / Staphylococcus epidermidis / Methicillin Resistance / Biofilms / Vancomycin Resistance Limits: Adult / Aged / Female / Humans / Infant / Male / Infant, Newborn Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2014 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR