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Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
Peixoto, Mariana Lima Perazzini; Santos, Dilvani Oliveira; Souza, Ivy de Castro Campos de; Neri, Eloah Christina Lyrio; Sequeira, Danielly Correa Moreira de; Luca, Paula Mello De; Borba, Cíntia de Moraes.
  • Peixoto, Mariana Lima Perazzini; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Bioquímica e Bioprospecção de Fungos. Laboratório de Taxonomia. Rio de Janeiro. BR
  • Santos, Dilvani Oliveira; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Bioquímica e Bioprospecção de Fungos. Laboratório de Taxonomia. Rio de Janeiro. BR
  • Souza, Ivy de Castro Campos de; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Bioquímica e Bioprospecção de Fungos. Laboratório de Taxonomia. Rio de Janeiro. BR
  • Neri, Eloah Christina Lyrio; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Bioquímica e Bioprospecção de Fungos. Laboratório de Taxonomia. Rio de Janeiro. BR
  • Sequeira, Danielly Correa Moreira de; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Bioquímica e Bioprospecção de Fungos. Laboratório de Taxonomia. Rio de Janeiro. BR
  • Luca, Paula Mello De; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Bioquímica e Bioprospecção de Fungos. Laboratório de Taxonomia. Rio de Janeiro. BR
  • Borba, Cíntia de Moraes; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Bioquímica e Bioprospecção de Fungos. Laboratório de Taxonomia. Rio de Janeiro. BR
Rev. Soc. Bras. Med. Trop ; 47(5): 613-617, Sep-Oct/2014. graf
Article in English | LILACS | ID: lil-728898
ABSTRACT
Introduction Purpureocillium lilacinum is emerging as a causal agent of hyalohyphomycosis that is refractory to antifungal drugs; however, the pathogenic mechanisms underlying P. lilacinum infection are not understood. In this study, we investigated the interaction of P. lilacinum conidia with human macrophages and dendritic cells in vitro. Methods Spores of a P. lilacinum clinical isolate were obtained by chill-heat shock. Mononuclear cells were isolated from eight healthy individuals. Monocytes were separated by cold aggregation and differentiated into macrophages by incubation for 7 to 10 days at 37°C or into dendritic cells by the addition of the cytokines human granulocyte-macrophage colony stimulating factor and interleukin-4. Conidial suspension was added to the human cells at 11, 21, and 51 (conidiacells) ratios for 1h, 6h, and 24h, and the infection was evaluated by Giemsa staining and light microscopy. Results After 1h interaction, P. lilacinum conidia were internalized by human cells and after 6h contact, some conidia became inflated. After 24h interaction, the conidia produced germ tubes and hyphae, leading to the disruption of macrophage and dendritic cell membranes. The infection rate analyzed after 6h incubation of P. lilacinum conidia with cells at 21 and 11 ratios was 76.5% and 25.5%, respectively, for macrophages and 54.3% and 19.5%, respectively, for cultured dendritic cells. Conclusions P. lilacinum conidia are capable of infecting and destroying both macrophages and dendritic cells, clearly demonstrating the ability of this pathogenic fungus to invade human phagocytic cells. .
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Full text: Available Index: LILACS (Americas) Main subject: Ascomycota / Dendritic Cells / Macrophages Limits: Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz/BR

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Full text: Available Index: LILACS (Americas) Main subject: Ascomycota / Dendritic Cells / Macrophages Limits: Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz/BR