Your browser doesn't support javascript.
loading
Evaluation of constitutive and inducible resistance to clindamycin in clinical samples of Staphylococcus aureus from a tertiary hospital
Bottega, Angelita; Rodrigues, Mônica de Abreu; Carvalho, Fernanda Aguirre; Wagner, Tatiana Feyh; Leal, Isabel Agne Souza; Santos, Silvana Oliveira dos; Rampelotto, Roberta Filipini; Hörner, Rosmari.
  • Bottega, Angelita; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
  • Rodrigues, Mônica de Abreu; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
  • Carvalho, Fernanda Aguirre; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
  • Wagner, Tatiana Feyh; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
  • Leal, Isabel Agne Souza; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
  • Santos, Silvana Oliveira dos; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
  • Rampelotto, Roberta Filipini; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
  • Hörner, Rosmari; Universidade Federal de Santa Maria. Departamento de Análises Clínicas e Toxicológicas. Santa Maria. BR
Rev. Soc. Bras. Med. Trop ; 47(5): 589-592, Sep-Oct/2014. tab
Article in English | LILACS | ID: lil-728903
ABSTRACT
Introduction Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have become common in hospitals and the community environment, and this wide resistance has limited patient treatment. Clindamycin (CL) represents an important alternative therapy for infections caused by S. aureus. Antimicrobial susceptibility testing using standard methods may not detect inducible CL resistance. This study was performed to detect the phenotypes of resistance to macrolides-lincosamides-streptogramin B (MLSB) antibiotics, including CL, in clinical samples of S. aureus from patients at a tertiary hospital in Santa Maria, State of Rio Grande do Sul, Brazil. Methods One hundred and forty clinical isolates were submitted to the disk diffusion induction test (D-test) with an erythromycin (ER) disk positioned at a distance of 20mm from a CL disk. The results were interpreted according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Results In this study, 29 (20.7%) of the 140 S. aureus samples were resistant to methicillin (MRSA), and 111 (79.3%) were susceptible to methicillin (MSSA). The constitutive resistance phenotype (cMLSB) was observed in 20 (14.3%) MRSA samples and in 5 (3.6%) MSSA samples, whereas the inducible resistance phenotype (iMLSB) was observed in 3 (2.1%) MRSA samples and in 8 (5.8%) MSSA samples. Conclusions The D-test is essential for detecting the iMLSB phenotype because the early identification of this phenotype allows clinicians to choose an appropriate treatment for patients. Furthermore, this test is simple, easy to perform and inexpensive. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Staphylococcus aureus / Clindamycin / Erythromycin / Anti-Bacterial Agents Type of study: Practice guideline Limits: Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Maria/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Staphylococcus aureus / Clindamycin / Erythromycin / Anti-Bacterial Agents Type of study: Practice guideline Limits: Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Maria/BR