Dried blood spot testing for the antenatal screening of HTLV, HIV, syphilis, toxoplasmosis and hepatitis B and C: prevalence, accuracy and operational aspects
Braz. j. infect. dis
;
18(6): 618-624, Nov-Dec/2014. tab
Article
in English
| LILACS
| ID: lil-730415
ABSTRACT
Introduction:
Screening for vertically transmitted infection is mandatory and must be conducted at the first prenatal consultation. The most vulnerable women's groups are those at the lowest socio-economic level. Dried blood spot testing on filter paper could represent a secure way to screen pregnant women in the prenatal period.Methods:
A cross-sectional study was conducted between November 2009 and March 2010, in the Metropolitan Region of Salvador, Bahia, Brazil, to compare the accuracy of the dried blood spot in filter paper and venipuncture serological as screening methods for HIV, HTLV, VHB, VHC, Treponema pallidum, and Toxoplasma gondii during prenatal period. Results of the venous blood sample collected in tubes were considered the gold standard.Results:
Serum samples and dried blood spot were obtained from 692 pregnant women aged between 14 and 42 years, with a median age of 26. Thirteen women were seropositive for T. gondii (1.88%; 95% CI 0.60–2.71%), five for T. pallidum (0.72%; 95% CI 0.15–1.61%), two for HBV (0.29%; 95% CI 0.050.95%) and one for HTLV-1 (0.14%; 95% CI 0.01–0.71%). No one was positive for HCV and HIV. The dried blood spot accuracy for syphilis and HTLV were 100% (95% CI 99.25–100) and 100% (95% CI 99.45–100%), respectively. The average time between blood collection and recording of the sample in the reference laboratory was 4.93 (3.82) days and between dried blood spot processing and active search for pregnant women was 3.44 (4.27) days.Conclusions:
The use of dried blood spot may represent a secure way to expedite access to results of vertically transmitted diseases in the prenatal period, particularly in regions with scarce healthcare resources. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Pregnancy Complications, Infectious
/
Dried Blood Spot Testing
Type of study:
Diagnostic study
/
Observational study
/
Prevalence study
/
Risk factors
/
Screening study
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Pregnancy
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Associação de Pais e Amigos dos Excepcionais de Salvador – APAE Salvador/BR
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