Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens
Braz. j. infect. dis
;
18(6): 600-608, Nov-Dec/2014. tab, graf
Article
in English
| LILACS
| ID: lil-730425
ABSTRACT
Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretory antigenic target 6 were used to develop a sandwich-form fluorescence resonance energy transfer-based biosensor to detect M. tuberculosis complex and differentiate M. tuberculosis and M. bovis Bacille Calmette–Guerin simultaneously. The sensitivity and specificity of the newly developed biosensor were 94.2% and 86.6%, respectively, while the sensitivity and specificity of polymerase chain reaction and nested polymerase chain reaction were considerably lower, 74.2%, 73.3% and 82.8%, 80%, respectively. The detection limits of the sandwich-form fluorescence resonance energy transfer-based biosensor were far lower (10 fg) than those of the polymerase chain reaction and nested polymerase chain reaction (100 fg). Although the cost of the developed nanobiosensor was slightly higher than those of the polymerase chain reaction-based techniques, its unique advantages in terms of turnaround time, higher sensitivity and specificity, as well as a 10-fold lower detection limit would clearly recommend this test as a more appropriate and cost-effective tool for large scale operations.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Sputum
/
Tuberculosis, Pulmonary
/
Biosensing Techniques
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2014
Type:
Article
Affiliation country:
Malaysia
Institution/Affiliation country:
Universiti Putra Malaysia/MY
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