Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury
Acta cir. bras
;
29(12): 812-818, 12/2014. tab, graf
Article
in English
| LILACS
| ID: lil-731027
ABSTRACT
PURPOSE:
To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury.METHODS:
Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and dex (given by dexmedetomidine) groups. Acute hyperglycemia was induced by intraperitoneal injection (i.p.) of 25% glucose (3 g/kg) 45 min before ischemia. Dexmedetomidine (50 μg/kg, i.p.) was administrated 30 min before induction of ischemia. Renal function, histology, apoptosis, expression of Bax, Bcl-2 and phosphorylated AKT (p-AKT) were detected.RESULTS:
I/R insult significantly increased the serum levels of blood urea nitrogen and creatinine, apoptotic tubular epithelial cells, expression of Bax and p-AKT, but decreased Bcl-2 expression. All these changes were further enhanced by hyperglycemia (p<0.05). In hyperglycemic condition, there was no statistically difference between the I/R group and Dex group in all the aforementioned detection indexes (p>0.05).CONCLUSION:
Acute hyperglycemia attenuates dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury in non-diabetic rats. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Reperfusion Injury
/
Ischemic Preconditioning
/
Dexmedetomidine
/
Hyperglycemia
/
Ischemia
/
Kidney
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2014
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Wuhan University/CN
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