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Effect of Super-Oxidized Water, Sodium Hypochlorite and EDTA on Dentin Microhardness
Ghisi, Alexandre Corrêa; Kopper, Patrícia Maria Poli; Baldasso, Flávia E. R.; Stürmer, Caroline P.; Rossi-Fedele, Giampiero; Steier, Liviu; Figueiredo, José Antonio Poli de; Morgental, Renata Dornelles; Vier-Pelisser, Fabiana Vieira.
  • Ghisi, Alexandre Corrêa; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Kopper, Patrícia Maria Poli; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Baldasso, Flávia E. R.; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Stürmer, Caroline P.; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Rossi-Fedele, Giampiero; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Steier, Liviu; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Figueiredo, José Antonio Poli de; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Morgental, Renata Dornelles; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
  • Vier-Pelisser, Fabiana Vieira; PUCRS - Pontifical Catholic University of Rio Grande do Sul. School of Dentistry. Department of Endodontics. Porto Alegre. BR
Braz. dent. j ; 25(5): 420-424, Sep-Oct/2014. tab
Article in English | LILACS | ID: lil-731056
ABSTRACT
The present study aimed to evaluate the influence of the following irrigating solutions on the microhardness of root canal dentin 2% sodium hypochlorite (2NaOCl), 5% sodium hypochlorite (5NaOCl), super-oxidized water (400 ppm Sterilox - Sx) and 17% EDTA (E). Eighty roots from bovine incisors were randomly divided into 8 groups (n=10) 2NaOCl, 5NaOCl, Sx, and 2NaOCl + E, 5NaOCl + E, Sx + E (associated with E as final irrigant for 5 min), E solely and distilled water (dH2O) as the negative control. Root canal preparation was performed by hand instruments, using one of the irrigation protocols for 30 min. Then, 5 mm of the cervical root third were cut out from each sample and subjected to the Vickers microhardness test, at two points, one at approximately 500-1000 µm from the root canal lumen (distance 1), and the other at approximately 500-1000 µm from the external root surface (distance 2). Data were analyzed by Wilcoxon and Kruskal-Wallis tests at 5% significance level. Microhardness values at distance 1 were significantly lower than those at distance 2 for all groups, except 5NaOCl and 5NaOCl + E groups (p>0.05). EDTA showed the lowest microhardness values. However, no statistically significant difference was detected among groups at distance 1 and EDTA was significantly different only from Sx at distance 2. In conclusion, all tested solutions showed lower microhardness at the most superficial root canal dentin layer compared to the one found near the external root surface, except 5NaOCl and 5NaOCl + E; EDTA promoted lower microhardness values in comparison to Sterilox at this site.
RESUMO
O presente estudo teve como objetivo avaliar a influência das seguintes soluções irrigadoras na microdureza da dentina do canal radicular hipoclorito de sódio a 2% (NaOCl2), hipoclorito de sódio a 5% (NaOCl5), água superoxidada (Sterilox(r) 400 ppm - Sx) e EDTA a 17% (E). Oitenta raízes de incisivos bovinos foram divididas aleatoriamente em 8 grupos (n=10) NaOCl2, NaOCl5, Sx e NaOCl2 + E, NaOCl5 + E, Sx + E (associados ao E como irrigante final por 5 min), E isolado e água destilada (H2Od), como controle negativo. O preparo dos canais radiculares foi realizado com instrumentos manuais, usando um dos protocolos de irrigação por 30 min. A seguir, 5 mm do terço cervical de cada amostra foram cortados perpendicularmente e submetidos ao teste de microdureza de Vickers, em dois pontos, um aproximadamente 500-1000 µm da luz do canal radicular (distância 1), e o outro aproximadamente 500-1000 µm da superfície externa da raiz (distância 2). Os dados foram analisados pelos testes de Wilcoxon e Kruskal-Wallis com um nível de significância de 5%. Os valores de microdureza na distância 1 foram significativamente menores do que na distância 2 para todos os grupos, exceto NaOCl5 e NaOCl5 +E (p>0,05). O EDTA mostrou os menores valores de microdureza. No entanto, não foi detectada diferença estatisticamente significativa entre os grupos na distância 1 e o EDTA foi significativamente diferente apenas do Sx na distância 2. Pode-se concluir que todas as soluções testadas mostraram menor microdureza na camada de dentina mais superficial do canal radicular em comparação aos valores encontrados próximo à superfície radicular externa, exceto NaOCl5 e NaOCl5 + E; o EDTA promoveu menor microdureza em comparação ao Sterilox(r) neste ponto.
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Full text: Available Index: LILACS (Americas) Main subject: Transcription Factors / Mouth Neoplasms / Carcinoma, Squamous Cell / Sulindac / Anti-Inflammatory Agents, Non-Steroidal / Receptors, Cytoplasmic and Nuclear / Antineoplastic Agents Type of study: Controlled clinical trial Limits: Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: PUCRS - Pontifical Catholic University of Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Transcription Factors / Mouth Neoplasms / Carcinoma, Squamous Cell / Sulindac / Anti-Inflammatory Agents, Non-Steroidal / Receptors, Cytoplasmic and Nuclear / Antineoplastic Agents Type of study: Controlled clinical trial Limits: Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2014 Type: Article Affiliation country: Brazil Institution/Affiliation country: PUCRS - Pontifical Catholic University of Rio Grande do Sul/BR