Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
Mem. Inst. Oswaldo Cruz
;
109(8): 1078-1080, 12/2014. tab, graf
Article
in English
| LILACS
| ID: lil-732594
ABSTRACT
Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Giardia lamblia
/
Microbial Viability
/
Metronidazole
/
Antiprotozoal Agents
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2014
Type:
Article
/
Project document
Affiliation country:
Portugal
Institution/Affiliation country:
Universidade do Porto/PT
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