Validación de una técnica de RT-PCR en tiempo real para la detección del virus de fiebre aftosa y evaluación de su desempeño en la infección aguda

Fondevila, Norberto; Compaired, Diego; Maradei, Eduardo; Duffy, Sergio

Validación de una técnica de RT-PCR en tiempo real para la detección del virus de fiebre aftosa y evaluación de su desempeño en la infección aguda / Validation of a real time RT-PCR assay to detect foot-and-mouth disease virus and assessment of its performance in acute infection

Fondevila, Norberto; Compaired, Diego; Maradei, Eduardo; Duffy, Sergio.

Fondevila, Norberto; INTA. EEA Castelar. CICVyA. Instituto de Virología. Hurlingham. AR
Compaired, Diego; INTA. EEA Castelar. CICVyA. Instituto de Virología. Hurlingham. AR
Maradei, Eduardo; INTA. EEA Castelar. CICVyA. Instituto de Virología. Hurlingham. AR
Duffy, Sergio; INTA. EEA Castelar. CICVyA. Instituto de Virología. Hurlingham. AR

Rev. argent. microbiol ; 46(3): 188-195, oct. 2014. graf, tab

Article in Spanish | LILACS | ID: lil-734581

Subject(s)

Animals; Cattle; Cattle Diseases/diagnosis; Foot-and-Mouth Disease Virus/isolation & purification; Foot-and-Mouth Disease/diagnosis; Real-Time Polymerase Chain Reaction/methods; Reverse Transcriptase Polymerase Chain Reaction/methods; Acute Disease; Body Fluids/virology; Cattle Diseases/virology; Esophagus/virology; Foot-and-Mouth Disease Virus/classification; Foot-and-Mouth Disease Virus/genetics; Foot-and-Mouth Disease Virus/immunology; Foot-and-Mouth Disease/virology; Pharynx/virology; Reproducibility of Results; RNA, Viral/analysis; Real-Time Polymerase Chain Reaction/instrumentation; Reverse Transcriptase Polymerase Chain Reaction/instrumentation; Sensitivity and Specificity; Specimen Handling; Vaccination/veterinary; Viral Vaccines/immunology

Diagnosis; Diagnóstico; Fiebre Aftosa; Foot-and-mouth disease; RT-PCRtr; Real time RT-PCR

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Full text: Available Index: LILACS (Americas) Main subject: Cattle Diseases / Reverse Transcriptase Polymerase Chain Reaction / Foot-and-Mouth Disease Virus / Real-Time Polymerase Chain Reaction / Foot-and-Mouth Disease Type of study: Diagnostic study Limits: Animals Language: Spanish Journal: Rev. argent. microbiol Journal subject: Microbiology Year: 2014 Type: Article / Project document Affiliation country: Argentina Institution/Affiliation country: INTA/AR

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