Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction
Acta cir. bras
;
30(1): 73-79, 01/2015. tab, graf
Article
in English
| LILACS
| ID: lil-735708
ABSTRACT
PURPOSE:
To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats.METHODS:
Sixteen male rats were allocated across four groups LG - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis.RESULTS:
There were no differences in bursting pressure among groups LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation.CONCLUSION:
Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Wound Healing
/
Cholestasis, Extrahepatic
/
Colon
/
Jaundice, Obstructive
/
Glutamine
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Brasilia University/BR
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