Sugar inhibition of the lectin jacalin: comparison of three assays
Braz. j. med. biol. res
;
22(5): 601-10, 1989. tab, ilus
Article
in English
| LILACS
| ID: lil-73900
RESUMO
1. Three assays were used to test nine sugars for inhibition of jacalin activity prepared from Artocarpus intefrifolia. Rat spleen proliferation was unssuitable since the measurement of the effects of sugars against jacalin binding was complicated by their simultaneous metavolic effects on the cells. 2. Based partly on a sheep red blood cell hemagglutination assay and mainly on human serum protein preciptation, the following potencies in relation to D(+)-galactose (taken as 1) were obtained: 1-0-methyl-alfa-D-galactopyranoside, 40; methyl-alfa-D-mannopyranoside and D(+)-mannose, 0.12; ß-Dd-(-)-fructose, 0.08; alfa - D(+)-glucose and 1 - 0-methyl-ß-D-glucopyranoside, <0.04
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Index:
LILACS (Americas)
Main subject:
Lectins
/
Monosaccharides
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1989
Type:
Article
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