Leishmania donovani donovani and Leishmania donovani chagasi as antigens in a direct agglutination assay for the diagnosis of kala-azar
Braz. j. med. biol. res
;
22(5): 611-5, 1989. ilus
Article
in English
| LILACS
| ID: lil-73901
RESUMO
The need for a reliable method for the immunological diagnosis of Kala-azar is imperative. Leishmania donovani donovani and L. donovani chagasi culture promastigotes were compared as antigens in a direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis in Brazil. Both antigens were successfully employed for the DAT, Showing 100½ sensitivity and > 98% specificity when used to test sera from Brasilian and African Kala-azar, Chagas'disease, malaria, filaria and symphilis patients, and on sera from Brazilian controls. Cross-reactions were sometimes observed when cutaneous and cucocutaneous leishmaniasis patient sera were teste. The vross-reactions were completely abolished by the addition of 0.78% 2-mercaptoethanol to the serum diluent. These data show data that this improved Dat can be used for the diagnosis of visceral leishmaniasis in Brazil
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Index:
LILACS (Americas)
Main subject:
Leishmania donovani
/
Leishmaniasis, Visceral
/
Antigens, Protozoan
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1989
Type:
Article
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