Pentoxifylline and prostaglandin E1 action on ischemia and reperfusion of small intestine tissue in rats. An immunohistochemical study
Acta cir. bras
;
30(2): 115-119, 02/2015. tab, graf
Article
in English
| LILACS
| ID: lil-741025
ABSTRACT
PURPOSE:
To investigate the action of pentoxifylline (PTX) and prostaglandin E1 (PGE1) on ischemia and reperfusion of small intestine tissue in rats, using immunohistochemical analysis.METHODS:
Thirty-five Wistar rats were distributed as follows group A (n=10) subjected to intestinal ischemia and reperfusion for 60 min, with no drugs; group B (n=10) PTX given during tissue ischemia and reperfusion; group C (n=10) PGE1 given during tissue ischemia and reperfusion; group D (n=5) sham. A segment of the small intestine was excised from each euthanized animal and subjected to immunohistochemical examination.RESULTS:
Mean number of cells expressing anti-FAS ligand in the crypts was highest in Group A (78.9 ± 17.3), followed by groups B (16.7 ± 2.8), C (11.3 ± 1.8), and D (2.5 ± 0.9), with very significant differences between groups (p<0.0001).CONCLUSIONS:
The use of pentoxifylline or prostaglandin E1 proved beneficial during tissue reperfusion. The immunohistochemical results demonstrated a decrease in apoptotic cells, while protecting other intestinal epithelium cells against death after reperfusion, allowing these cells to renew the epithelial tissue. .
Full text:
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Index:
LILACS (Americas)
Main subject:
Pentoxifylline
/
Vasodilator Agents
/
Alprostadil
/
Reperfusion Injury
/
Intestine, Small
/
Ischemia
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Mato Grosso do Sul/BR
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