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Production and characterization of an extracellular lipase from Candida guilliermondii
Oliveira, Anne Caroline Defranceschi; Fernandes, Maria Luiza; Mariano, André Bellin.
  • Oliveira, Anne Caroline Defranceschi; Universidade Federal do Paraná. Setor de Tecnologia, Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável. Curitiba. BR
  • Fernandes, Maria Luiza; Universidade Federal do Paraná. Setor de Tecnologia, Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável. Curitiba. BR
  • Mariano, André Bellin; Universidade Federal do Paraná. Setor de Tecnologia, Núcleo de Pesquisa e Desenvolvimento de Energia Autossustentável. Curitiba. BR
Braz. j. microbiol ; 45(4): 1503-1511, Oct.-Dec. 2014. ilus, graf, tab
Article in English | LILACS | ID: lil-741306
ABSTRACT
Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL-1) in the presence of 5 mmol L-1 NaCl at 30 ºC and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL-1. The produced enzyme was applied in the oleic acid esterification under different solvents hexane, cyclohexane or cyclohexanone) and different acidalcohol molar ratios. Higher ester conversion rate (81%) was obtained using hexane and the molar ratio of 19 was the best conditions using methanol. The results suggest the potential for development of endophytic yeast in the production of biocatalyst through submerged fermentation using agroindustrial residues as culture medium.
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Full text: Available Index: LILACS (Americas) Main subject: Candida / Lipase Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2014 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Paraná/BR

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Full text: Available Index: LILACS (Americas) Main subject: Candida / Lipase Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2014 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Paraná/BR