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Genetic variability in G2 and F2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure
Moraes, Claudia Trigo Pedroso; Oliveira, Danielle Bruna Leal; Campos, Angelica Cristine Almeida; Bosso, Patricia Alves; Lima, Hildener Nogueira; Stewien, Klaus Eberhard; Gilio, Alfredo Elias; Vieira, Sandra Elisabete; Botosso, Viviane Fongaro; Durigon, Edison Luiz.
  • Moraes, Claudia Trigo Pedroso; Instituto de Ciências Biomédicas.
  • Oliveira, Danielle Bruna Leal; Instituto de Ciências Biomédicas.
  • Campos, Angelica Cristine Almeida; Instituto de Ciências Biomédicas.
  • Bosso, Patricia Alves; Instituto de Ciências Biomédicas.
  • Lima, Hildener Nogueira; Instituto de Ciências Biomédicas.
  • Stewien, Klaus Eberhard; Instituto de Ciências Biomédicas.
  • Gilio, Alfredo Elias; Instituto de Ciências Biomédicas.
  • Vieira, Sandra Elisabete; Instituto de Ciências Biomédicas.
  • Botosso, Viviane Fongaro; Instituto de Ciências Biomédicas.
  • Durigon, Edison Luiz; Instituto de Ciências Biomédicas.
Mem. Inst. Oswaldo Cruz ; 110(1): 138-141, 03/02/2015. tab
Article in English | LILACS | ID: lil-741607
ABSTRACT
Human respiratory syncytial virus (HRSV) is an important respiratory pathogens among children between zero-five years old. Host immunity and viral genetic variability are important factors that can make vaccine production difficult. In this work, differences between biological clones of HRSV were detected in clinical samples in the absence and presence of serum collected from children in the convalescent phase of the illness and from their biological mothers. Viral clones were selected by plaque assay in the absence and presence of serum and nucleotide sequences of the G2 and F2 genes of HRSV biological clones were compared. One non-synonymous mutation was found in the F gene (Ile5Asn) in one clone of an HRSV-B sample and one non-synonymous mutation was found in the G gene (Ser291Pro) in four clones of the same HRSV-B sample. Only one of these clones was obtained after treatment with the child's serum. In addition, some synonymous mutations were determined in two clones of the HRSV-A samples. In conclusion, it is possible that minor sequences could be selected by host antibodies contributing to the HRSV evolutionary process, hampering the development of an effective vaccine, since we verify the same codon alteration in absence and presence of human sera in individual clones of BR-85 sample.
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Full text: Available Index: LILACS (Americas) Main subject: Oils, Volatile / Cocos / Silicon Dioxide / Crops, Agricultural / Pelargonium / Monoterpenes / Aluminum Oxide / Fruit Country/Region as subject: Asia Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article

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Full text: Available Index: LILACS (Americas) Main subject: Oils, Volatile / Cocos / Silicon Dioxide / Crops, Agricultural / Pelargonium / Monoterpenes / Aluminum Oxide / Fruit Country/Region as subject: Asia Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article