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Experimental infection with the Toxoplasma gondii ME-49 strain in the Brazilian BR-1 mini pig is a suitable animal model for human toxoplasmosis
Miranda, Farlen José Bebber; Souza, Diogo Benchimol de; Frazão-Teixeira, Edwards; Oliveira, Fábio Conceição de; Melo, João Cardoso de; Mariano, Carlos Magno Anselmo; Albernaz, Antonio Peixoto; Carvalho, Eulógio Carlos Queiróz de; Oliveira, Francisco Carlos Rodrigues de; Souza, Wanderley de; DaMatta, Renato Augusto.
  • Miranda, Farlen José Bebber; Laboratório de Biologia Celular e Tecidual.
  • Souza, Diogo Benchimol de; Laboratório de Biologia Celular e Tecidual.
  • Frazão-Teixeira, Edwards; Laboratório de Biologia Celular e Tecidual.
  • Oliveira, Fábio Conceição de; Laboratório de Biologia Celular e Tecidual.
  • Melo, João Cardoso de; Laboratório de Biologia Celular e Tecidual.
  • Mariano, Carlos Magno Anselmo; Laboratório de Biologia Celular e Tecidual.
  • Albernaz, Antonio Peixoto; Laboratório de Biologia Celular e Tecidual.
  • Carvalho, Eulógio Carlos Queiróz de; Laboratório de Biologia Celular e Tecidual.
  • Oliveira, Francisco Carlos Rodrigues de; Laboratório de Biologia Celular e Tecidual.
  • Souza, Wanderley de; Laboratório de Biologia Celular e Tecidual.
  • DaMatta, Renato Augusto; Laboratório de Biologia Celular e Tecidual.
Mem. Inst. Oswaldo Cruz ; 110(1): 95-100, 03/02/2015. tab, graf
Article in English | LILACS | ID: lil-741615
ABSTRACT
Toxoplasma gondii causes toxoplasmosis, a worldwide disease. Experimentation with pigs is necessary for the development of new therapeutic approaches to human diseases. BR-1 mini pigs were intramuscularly infected with T. gondii with tachyzoites (RH strain) or orally infected with cysts (ME-49 strain). Haematology and serum biochemistry were analysed and buffy coat cells were inoculated in mice to determine tachyzoite circulation. No alterations were observed in erythrocyte and platelet values; however, band neutrophils increased seven days after infection with ME-49. Serology of the mice inoculated with pig blood leucocytes revealed circulating ME-49 or RH strain tachyzoites in the pigs' peripheral blood at two and seven or nine days post-infection. The tachyzoites were also directly observed in blood smears from the infected pigs outside and inside leucocytes for longer periods. Alanine-aminotransferase was high at days 21 and 32 in the RH infected pigs. After 90 days, the pigs were euthanised and their tissue samples were processed and inoculated into mice. The mice serology revealed the presence of parasites in the hearts, ileums and mesenteric lymph nodes of the pigs. Additionally, cysts in the mice were only observed after pig heart tissue inoculation. The infected pigs presented similar human outcomes with relatively low pathogenicity and the BR-1 mini pig model infected with ME-49 is suitable to monitor experimental toxoplasmosis.
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Full text: Available Index: LILACS (Americas) Main subject: Seeds / Calcium, Dietary / Food Quality / Food Inspection / Phosphorus, Dietary / Iron, Dietary / Chenopodium quinoa Limits: Humans Country/Region as subject: South America / Brazil / Chile Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article

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Full text: Available Index: LILACS (Americas) Main subject: Seeds / Calcium, Dietary / Food Quality / Food Inspection / Phosphorus, Dietary / Iron, Dietary / Chenopodium quinoa Limits: Humans Country/Region as subject: South America / Brazil / Chile Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article