Renal biomarkers of male and female Wistar rats (Rattus norvegicus) undergoing renal ischemia and reperfusion
Acta cir. bras
;
30(4): 277-288, 04/2015. tab, graf
Article
in English
| LILACS
| ID: lil-744279
ABSTRACT
PURPOSE:
To investigate biomarkers of acute renal injury in Wistar rats, subjected to left renal ischemia for 10 minutes, and then compare reperfusion at 24 hours, and at 5, 7, 14 and 21 days after the procedure.METHODS:
Eight female and male rats between 60 and 81 days old were used in the Central Animal Facility of the UFMS. Assessed biomarkers included urine protein, urea, creatinine, glucose, sodium, potassium, urine alkaline phosphatase and gamma-glutamyl transferase activities, and protein-to-creatinine ratio; and in serum urea, creatinine, sodium and potassium, fractional excretion of sodium, potassium, urine flow and creatinine clearance.RESULTS:
Greater variance was observed in the parameters at 24 hours and at five days (p<0.05) after reperfusion. On the 21st day, these parameters approximated those obtained for the control group.CONCLUSIONS:
Renal ischemia for 10 minutes was sufficient to raise urine levels of protein, glucose, fractional excretion of potassium, urea, creatinine clearance, urine activity of gamma-glutamyltransferase and alkaline phosphatase enzymes in the first 24 hours, up to five days after reperfusion, which may indicate risk of acute kidney injury, according to the RIFLE classification. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Biomarkers
/
Reperfusion Injury
/
Acute Kidney Injury
/
Ischemia
/
Kidney
Type of study:
Etiology study
/
Risk factors
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2015
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
UFMS/BR
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