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Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats?
Çakır, Tuğrul; Baştürk, Ahmet; Polat, Cemal; Aslaner, Arif; Durgut, Himmet; Şehirli, Ahmet Özer; Gül, Mehmet; Öğünç, Ayliz Velioğlu; Gül, Semir; Sabuncuoglu, Mehmet Zafer; Oruç, Mehmet Tahir.
  • Çakır, Tuğrul; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Baştürk, Ahmet; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Polat, Cemal; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Aslaner, Arif; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Durgut, Himmet; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Şehirli, Ahmet Özer; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Gül, Mehmet; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Öğünç, Ayliz Velioğlu; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Gül, Semir; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Sabuncuoglu, Mehmet Zafer; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
  • Oruç, Mehmet Tahir; Antalya Training and Research Hospital. Department of General Surgery. Antalya. TR
Acta cir. bras ; 30(4): 247-252, 04/2015. tab, graf
Article in English | LILACS | ID: lil-744281
ABSTRACT

PURPOSE:

To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats.

METHODS:

Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-α, IL-1β, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination.

RESULTS:

MTX administration caused a significant decrease in tissue GSH, and tissue Na+, K+ ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-α, IL- β) were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX.

CONCLUSION:

Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Methotrexate / Thioctic Acid / Chemical and Drug Induced Liver Injury / Antimetabolites, Antineoplastic / Antioxidants Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2015 Type: Article Affiliation country: Turkey Institution/Affiliation country: Antalya Training and Research Hospital/TR

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Full text: Available Index: LILACS (Americas) Main subject: Methotrexate / Thioctic Acid / Chemical and Drug Induced Liver Injury / Antimetabolites, Antineoplastic / Antioxidants Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2015 Type: Article Affiliation country: Turkey Institution/Affiliation country: Antalya Training and Research Hospital/TR