Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats?
Acta cir. bras
;
30(4): 247-252, 04/2015. tab, graf
Article
in English
| LILACS
| ID: lil-744281
ABSTRACT
PURPOSE:
To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats.METHODS:
Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-α, IL-1β, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination.RESULTS:
MTX administration caused a significant decrease in tissue GSH, and tissue Na+, K+ ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-α, IL- β) were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX.CONCLUSION:
Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Methotrexate
/
Thioctic Acid
/
Chemical and Drug Induced Liver Injury
/
Antimetabolites, Antineoplastic
/
Antioxidants
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2015
Type:
Article
Affiliation country:
Turkey
Institution/Affiliation country:
Antalya Training and Research Hospital/TR
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