CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell
Rev. bras. hematol. hemoter
;
37(2): 98-102, Mar-Apr/2015. tab, graf
Article
in English
| LILACS
| ID: lil-746095
ABSTRACT
Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of con- sensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. Objective:
The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells.Methods:
Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies.Results:
In spite of the rarity of the events, they were detectable and presented similar interperson numbers of circulating endothelial cells.Conclusion:
The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Endothelial Cells
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Rev. bras. hematol. hemoter
Journal subject:
Hematology
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Campinas/BR
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