Comparison of recombinant A2-ELISA with rKE16 dipstick and direct agglutination tests for diagnosis of visceral leishmaniasis in dogs in Northwestern Iran
Rev. Soc. Bras. Med. Trop
;
48(2): 188-193, mar-apr/2015. tab, graf
Article
in English
| LILACS
| ID: lil-746220
ABSTRACT
INTRODUCTION:
Various methods are used for the diagnosis of visceral leishmaniasis (VL), such as microscopic examination, culture and inoculation of laboratory animals; however, serological assays are commonly used for the detection of antibodies in serum samples with a wide range of specificity and sensitivity.METHODS:
The purpose of this study was to compare three serological methods, including rA2-ELISA, the recombinant KE16 (rKE16) dipstick test and the direct agglutination test (DAT), for the detection of antibodies against VL antigens. The assays utilized 350 statistically based random serum samples from domestic dogs with clinical symptoms as well as samples from asymptomatic and healthy dogs from rural and urban areas of the Meshkinshahr district, northwestern Iran.RESULTS:
Samples were assessed, and the following positive rates were obtained 11.5% by rKE16, 26.9% by DAT and 49.8% by ELISA. The sensitivity among symptomatic dogs was 32.4% with rKE16, 100% with DAT and 52.9% with ELISA. Conversely, rA2-ELISA was less specific for asymptomatic dogs, at 46.5%, compared with DAT, at 88.9%.CONCLUSIONS:
This study recommends rA2-ELISA as a parallel assay combined with DAT to detect VL infection among dogs. Further evaluations should be performed to develop an inexpensive and reliable serologic test for the detection of Leishmania infantum among infected dogs. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Leishmania infantum
/
Dog Diseases
/
Leishmaniasis, Visceral
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2015
Type:
Article
/
Project document
Affiliation country:
Iran
Institution/Affiliation country:
Pasteur Institute of Iran/IR
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