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Expression of epithelial-mesenchymal transition markers at the invasive front of oral squamous cell carcinoma
COSTA, Liana Cristina Melo Carneiro; LEITE, Camila Ferreira; CARDOSO, Sérgio Vitorino; LOYOLA, Adriano Mota; FARIA, Paulo Rogério de; SOUZA, Paulo Eduardo Alencar; HORTA, Martinho Campolina Rebello.
  • COSTA, Liana Cristina Melo Carneiro; Pontifícia Universidade Católica de Minas Gerais. Department of Dentistry. Belo Horizonte. BR
  • LEITE, Camila Ferreira; Pontifícia Universidade Católica de Minas Gerais. Department of Dentistry. Belo Horizonte. BR
  • CARDOSO, Sérgio Vitorino; Pontifícia Universidade Católica de Minas Gerais. Department of Dentistry. Belo Horizonte. BR
  • LOYOLA, Adriano Mota; Pontifícia Universidade Católica de Minas Gerais. Department of Dentistry. Belo Horizonte. BR
  • FARIA, Paulo Rogério de; Pontifícia Universidade Católica de Minas Gerais. Department of Dentistry. Belo Horizonte. BR
  • SOUZA, Paulo Eduardo Alencar; Pontifícia Universidade Católica de Minas Gerais. Department of Dentistry. Belo Horizonte. BR
  • HORTA, Martinho Campolina Rebello; Pontifícia Universidade Católica de Minas Gerais. Department of Dentistry. Belo Horizonte. BR
J. appl. oral sci ; 23(2): 169-178, Mar-Apr/2015. tab, graf
Article in English | LILACS, BBO | ID: lil-746541
ABSTRACT
Oral squamous cell carcinoma (OSCC) is one of the most common malignances. In epithelial-mesenchymal transition (EMT), epithelial cells switch to mesenchymal-like cells exhibiting high mobility. This migratory phenotype is significant during tumor invasion and metastasis.

Objective:

The aim of this study is to evaluate the expression of the EMT markers E-cadherin, N-cadherin and vimentin in OSCC. Material and

Methods:

Immunohistochemical detection of E-cadherin, N-cadherin and vimentin was performed on 20 OSCC samples. Differences in the expression of each protein at the invasive front (IF) and in the central/superficial areas (CSA) of the tumor were assessed. Differences in the expression of each protein at the IF of both histologically high- and low-invasive OSCCs were evaluated. Associations among expression of proteins at the IF were assessed. Correlations between the expression levels of each protein at the IF and the tumor stage and clinical nodal status were also evaluated.

Results:

Reduced expression of E-cadherin was detected in 15 samples (75%). E-cadherin expression was reduced at the IF when compared to the CSA and in high-invasive tumors when compared to low-invasive tumors. All samples were negative for N-cadherin, even though one sample showed an inconspicuous expression. Positive expression of vimentin was observed in 6 samples (30%). Nevertheless, there was no difference in vimentin expression between the IF and the CSA regions or between the low- and high-invasive tumors. Furthermore, no association was observed among protein expression levels at the IF. Finally, no correlations were observed between each protein’s expression levels and tumor stage or clinical nodal status.

Conclusions:

Reduced E-cadherin expression at the IF and its association with histological invasiveness suggest that this protein is a noteworthy EMT marker in OSCC. Although vimentin was also detected as an EMT marker, its expression was ...
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Full text: Available Index: LILACS (Americas) Main subject: Endosomes / Molecular Chaperones / Microfilament Proteins Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pontifícia Universidade Católica de Minas Gerais/BR

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Full text: Available Index: LILACS (Americas) Main subject: Endosomes / Molecular Chaperones / Microfilament Proteins Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pontifícia Universidade Católica de Minas Gerais/BR