Minimally traumatic alveolar ridge augmentation with a tunnel injectable thermo-sensitive alginate scaffold
J. appl. oral sci
;
23(2): 215-223, Mar-Apr/2015. graf
Article
in English
| LILACS, BBO
| ID: lil-746543
ABSTRACT
Injectable bone substitutes and techniques have been developed for use in minimally invasive procedures for bone augmentation. Objective:
To develop a novel injectable thermo-sensitive alginate hydrogel (TSAH) as a scaffold to induce bone regeneration, using a minimally invasive tunnelling technique. Material andMethods:
An injectable TSAH was prepared from a copolymer solution of 8.0 wt% Poly(N-isopropylacrylamide) (PNIPAAm) and 8.0 wt% AAlg-g-PNIPAAm. In vitro properties of the material, such as its microstructure and the sustained release of recombinant human bone morphogenetic protein-2 (rhBMP-2), were investigated. Then, with the subperiosteal tunnelling technique, this material, carrying rhBMP-2, was injected under the labial periosteum of the maxillary anterior alveolar ridge in a rabbit model. New bone formation was evaluated by means of X-ray, micro-computed tomography (micro-CT), fluorescence labelling, histological study, and immunohistochemistry study.Results:
The material exhibited good injectability and thermo-irreversible properties. SEM showed an interconnected porous microstructure of the TSAH. The result of ALP activity indicated sustained delivery of BMP-2 from the TSAH from days 3 to 15. In a rabbit model, both TSAH and TSAH/rhBMP-2 induced alveolar ridge augmentation. The percentage of mineralised tissue in the TSAH/rhBMP-2 group (41.6±3.79%) was significantly higher than in the TSAH group (31.3±7.21%; p<0.05). The density of the regenerating tissue was higher in the TSAH/rhBMP-2 group than in the other groups (TSAH group, positive control, blank control; p<0.05).Conclusions:
The TSAH provided convenient handling properties for clinical application. To some extent, TSAH could induce ridge augmentation and mineral deposition, which can be enhanced when combined with rhBMP-2 for a minimally invasive tunnelling injection. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Brain
/
Brain Injuries
/
Receptors, Interleukin-1
/
Interleukin 1 Receptor Antagonist Protein
Type of study:
Controlled clinical trial
/
Diagnostic study
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2015
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Peking University School/CN
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