Effects of L-arginine and L-NAME on ischemia-reperfusion in rat liver
Acta cir. bras
;
30(5): 345-352, 05/2015. tab, graf
Article
in English
| LILACS
| ID: lil-747028
ABSTRACT
PURPOSE:
To evaluated the effects of L-arginine (a NO donor) and L-NAME (Nw-nitro-L-arginine methyl ester - a NOS inhibitor) on ischemia-reperfusion in rat livers.METHODS:
One hundred fifty two male Wistar rats were divided into four groups control (simulated surgery); hepatic IR; pretreatment with L-arginine plus hepatic IR; and L-NAME plus hepatic IR. The hepatocellular damage was evaluated at the first, third and seventh days after the procedures through the alanine-aminotransferase (ALT) and aspartate-aminotransaminase (AST) levels, as well as histopathological features vascular congestion (VC); steatosis (STE); necrosis (NEC); and inflammatory infiltration (INF). The mortality rate was also evaluated.RESULTS:
The pretreatment with L-NAME significantly worsened the AST levels after hepatic IR (p<0.05) at first day and L-arginine demonstrated an attenuating effect on ALT levels at seventh day (p<0.05). Furthermore, the administration of L-arginine was able to reduce the VC and STE in the seventh day after hepatic IR (p<0.05). The analysis of the mortality rates did not demonstrate any difference between the groups. Nevertheless, there was not effect of L-arginine and L-NAME on the mortality of the animals.CONCLUSION:
L-arginine/NO pathway has a role in the hepatic IR because the pretreatment with L-arginine partially had attenuated the hepatocellular damage induced by hepatic IR in rats. .
Full text:
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Index:
LILACS (Americas)
Main subject:
Arginine
/
Reperfusion Injury
/
NG-Nitroarginine Methyl Ester
/
Enzyme Inhibitors
/
Liver
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Health Sciences of Porto Alegre/BR
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