A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Braz. j. microbiol
;
46(1): 113-116, 05/2015. tab, graf
Article
in English
| LILACS
| ID: lil-748237
ABSTRACT
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Colorimetry
/
High-Throughput Screening Assays
/
Aminohydrolases
/
Hydro-Lyases
Type of study:
Diagnostic study
/
Evaluation studies
/
Screening study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual Paulista “Julio de Mesquita Filho”/BR
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