A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
Mem. Inst. Oswaldo Cruz
;
110(4): 573-576, 09/06/2015. tab, graf
Article
in English
| LILACS
| ID: lil-748860
ABSTRACT
We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochrome b-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plasmodium falciparum
/
Plasmodium vivax
/
Real-Time Polymerase Chain Reaction
/
Insect Vectors
/
Anopheles
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2015
Type:
Article
/
Project document
Affiliation country:
United States
Institution/Affiliation country:
New York State Department of Health/US
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