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KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
Biberg, Camila Arguelo; Rodrigues, Ana Claudia Souza; Carmo, Sidiane Ferreira do; Chaves, Claudia Elizabeth Volpe; Gales, Ana Cristina; Chang, Marilene Rodrigues.
  • Biberg, Camila Arguelo; Universidade Federal de Mato Grosso do Sul. Laboratório de Pesquisas Microbiológicas. Campo Grande. BR
  • Rodrigues, Ana Claudia Souza; Universidade Federal de Mato Grosso do Sul. Laboratório de Pesquisas Microbiológicas. Campo Grande. BR
  • Carmo, Sidiane Ferreira do; Universidade Federal de Mato Grosso do Sul. Laboratório de Pesquisas Microbiológicas. Campo Grande. BR
  • Chaves, Claudia Elizabeth Volpe; Universidade Federal de Mato Grosso do Sul. Laboratório de Pesquisas Microbiológicas. Campo Grande. BR
  • Gales, Ana Cristina; Universidade Federal de Mato Grosso do Sul. Laboratório de Pesquisas Microbiológicas. Campo Grande. BR
  • Chang, Marilene Rodrigues; Universidade Federal de Mato Grosso do Sul. Laboratório de Pesquisas Microbiológicas. Campo Grande. BR
Braz. j. microbiol ; 46(2): 501-504, Apr-Jun/2015. tab, graf
Article in English | LILACS | ID: lil-749737
ABSTRACT
The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Beta-Lactamases / Klebsiella Infections / Carbapenems / Klebsiella pneumoniae / Anti-Bacterial Agents Type of study: Practice guideline Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Mato Grosso do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Beta-Lactamases / Klebsiella Infections / Carbapenems / Klebsiella pneumoniae / Anti-Bacterial Agents Type of study: Practice guideline Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Mato Grosso do Sul/BR