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Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections
Suliman Al-Badah, Abdulhakim; S.S. Ibrahim, Abdelnasser; Al-Salamah, Ali A; Ibrahim, Shebl Salah S.
  • Suliman Al-Badah, Abdulhakim; King Saud University. College of Dentistry. Riyadh. SA
  • S.S. Ibrahim, Abdelnasser; King Saud University. College of Dentistry. Riyadh. SA
  • Al-Salamah, Ali A; King Saud University. College of Dentistry. Riyadh. SA
  • Ibrahim, Shebl Salah S; King Saud University. College of Dentistry. Riyadh. SA
Electron. j. biotechnol ; 18(3): 175-180, May 2015. ilus, graf, tab
Article in English | LILACS | ID: lil-750644
ABSTRACT
Background Enterococcus faecalis is considered to be one of most prevalent species in the oral cavity, particularly in endodontic infections. The aim of the present study was to investigate the prevalence of E. faecalis in dental root canals, clonal diversity by restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD-PCR) analysis, and the antibiotic susceptibility of E. faecalis isolates. Results Among the bacterial strains isolated from dental root canal specimens (n = 82), E. faecalis was determined to have the highest prevalence followed by Streptococcus viridians, Leuconostoc mesenteroides, Staphylococcus aureus, Streptococcus mitis, and Pediococcus pentosaceus. Cluster analysis of RAPD-PCR and RFLP patterns of the E. faecalis isolates discriminated five and six different genotypes, respectively. Among the tested strains, 43%, 52% and 5% were susceptible, intermediate resistant, and resistant to erythromycin, respectively. In addition, one strain (E-12) was intermediate resistant to linezolid, and one isolate (E-16) was resistant to tetracycline. Interestingly, many of the intermediate resistant/resistant strains were grouped in clusters 5 and 6, according RAPD and to RFLP, respectively. Conclusions E. faecalis demonstrated the highest prevalence in the tested dental root canal specimens collected from Saudi patients and were grouped into five to six different genotypes. Different levels of antimicrobial susceptibility were observed in the tested E. faecalis strains, which clearly indicated that although bacterial strains may be similar, point mutations can result in extreme susceptibility or resistance to various antibiotics. This phenomenon is a cause for concern for clinicians in the treatment of dental infections caused by E. faecalis.
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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Infections / Enterococcus faecalis / Drug Resistance, Bacterial / Dental Pulp Diseases Type of study: Risk factors Limits: Adolescent / Adult / Aged / Female / Humans / Male Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2015 Type: Article Affiliation country: Saudi Arabia Institution/Affiliation country: King Saud University/SA

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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Infections / Enterococcus faecalis / Drug Resistance, Bacterial / Dental Pulp Diseases Type of study: Risk factors Limits: Adolescent / Adult / Aged / Female / Humans / Male Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2015 Type: Article Affiliation country: Saudi Arabia Institution/Affiliation country: King Saud University/SA