Your browser doesn't support javascript.
loading
Evaluation of the effectiveness of packed red blood cell irradiation by a linear accelerator
Olivo, Ricardo Aparecido; Silva, Marcus Vinícius da; Garcia, Fernanda Bernadelli; Soares, Sheila; Rodrigues Junior, Virmondes; Moraes-Souza, Helio.
  • Olivo, Ricardo Aparecido; Universidade Federal do Triângulo Mineiro. Uberaba. BR
  • Silva, Marcus Vinícius da; Universidade Federal do Triângulo Mineiro. Uberaba. BR
  • Garcia, Fernanda Bernadelli; Universidade Federal do Triângulo Mineiro. Uberaba. BR
  • Soares, Sheila; Universidade Federal do Triângulo Mineiro. Uberaba. BR
  • Rodrigues Junior, Virmondes; Universidade Federal do Triângulo Mineiro. Uberaba. BR
  • Moraes-Souza, Helio; Universidade Federal do Triângulo Mineiro. Uberaba. BR
Rev. bras. hematol. hemoter ; 37(3): 153-159, May-Jun/2015. tab, graf
Article in English | LILACS | ID: lil-752538
ABSTRACT
Irradiation of blood components with ionizing radiation generated by a specific device is recommended to prevent transfusion-associated graft-versus-host disease. However, a lin- ear accelerator can also be used in the absence of such a device, which is the case of the blood bank facility studied herein. In order to evaluate the quality of the irradiated packed red blood cells, this study aimed to determine whether the procedure currently employed in the facility is effective in inhibiting the proliferation of T lymphocytes without damaging blood components. The proliferation of T lymphocytes, plasma potassium levels, and the degree of hemolysis were evaluated and compared to blood bags that received no irradiation. Packed red blood cell bags were irradiated at a dose of 25 Gy in a linear accelerator. For this purpose, a container was designed to hold the bags and to ensure even distribution of irradiation as evaluated by computed tomography and dose-volume histogram. Irradiation was observed to inhibit the proliferation of lymphocytes. The percentage of hemolysis in irradiated bags was slightly higher than in non-irradiated bags (p-value >0.05), but it was always less than 0.4% of the red cell mass. Although potassium increased in both groups, it was more pronounced in irradiated red blood cells, especially after seven days of storage, with a linear increase over storage time. The findings showed that, at an appropriate dosage and under validated conditions, the irradiation of packed red blood cells in a linear accelerator is effective, inhibiting lymphocyte proliferation but without compromising the viability of the red cells.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Particle Accelerators / Radiation / Radiation, Ionizing / T-Lymphocytes / Erythrocytes / Hemotherapy Service / Blood Safety Language: English Journal: Rev. bras. hematol. hemoter Journal subject: Hematology Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Triângulo Mineiro/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Particle Accelerators / Radiation / Radiation, Ionizing / T-Lymphocytes / Erythrocytes / Hemotherapy Service / Blood Safety Language: English Journal: Rev. bras. hematol. hemoter Journal subject: Hematology Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Triângulo Mineiro/BR