Gas Chromatography - Mass Spectrometry Analysis and Antibacterial Activity of Bluish-Green Pigment from Pseudomonas sp. JJTBVK (KF836502)
Braz. arch. biol. technol
;
58(4): 628-635, Jul-Aug/2015. tab, graf
Article
in English
| LILACS
| ID: lil-753948
ABSTRACT
The present study was conducted for the isolation of potential bacteria from the desert soil, their molecular identification and prediction of restriction sites of the potential isolate using the bioinformatics tools. Production of the metabolites was done by inoculating in nutrient broth of pH 8.6. Metabolite was bluish-green in color; it was extracted and dried by using methanol and used for partial characterization by using GC-MS spectroscopy. Antibacterial activity was performed with the clinical human pathogenic isolates. The bacterium was identified as Pseudomonas sp. JJTBVK on the basis of 16S rRNA sequencing. The sequence was analyzed for the restriction cleavage sites, which showed that the sequence had various restriction sites for different enzymes. Antibacterial activity (MIC) of methanol extract of the bacterial culture broth showed antibacterial activity (MIC), which was 29, 30, 30 and 29 mm for Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Salmonella typhi, respectively. GC-MS analysis of the methanol extract showed the presence of naphth [2,3-B] azet-2 (1H) -one, 1-phenyl-, which was the characteristic compound showing the antibacterial activity.
Full text:
Available
Index:
LILACS (Americas)
Type of study:
Prognostic study
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2015
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Shri JJT University/IN
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