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Effect of remote ischemic preconditioning in the expression of IL-6 and IL-10 in a rat model of liver ischemia-reperfusion injury
Guimarães Filho, Marco Antonio Correa; Cortez, Erika; Garcia-Souza, Érica Patrícia; Soares, Vivian de Melo; Moura, Aníbal Sanchez; Carvalho, Laís; Maya, Maria Cristina de Araujo; Pitombo, Marcos Bettini.
  • Guimarães Filho, Marco Antonio Correa; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
  • Cortez, Erika; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
  • Garcia-Souza, Érica Patrícia; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
  • Soares, Vivian de Melo; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
  • Moura, Aníbal Sanchez; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
  • Carvalho, Laís; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
  • Maya, Maria Cristina de Araujo; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
  • Pitombo, Marcos Bettini; Universidade Estadual do Rio de Janeiro. Department of General Surgery. BR
Acta cir. bras ; 30(7): 452-460, 07/2015. graf
Article in English | LILACS | ID: lil-754977
ABSTRACT

PURPOSE:

To study the effect of remote ischemic preconditioning (RIPC) in ischemia-reperfusion (I/R) liver injury and in the expression of IL-6 and IL-10 in a rat model.

METHODS:

Thirty-six male rats were divided in three groups Sham; I/R injury, a 45 minutes lobar liver ischemia and reperfusion; and RIPC, six cycles of four minutes of ischemia and four minutes of reperfusion on the right hindlimb followed by a 45 minutes lobar liver ischemia and reperfusion. Tissue and blood samples were collected after 1h and 3h of reperfusion for histopathological study, plasma cytokines and alanine aminotransferase (ALT) measurement.

RESULTS:

The histopathological study demonstrated a significant reduction in liver necrosis in the RIPC group (p<0,001). The ALT levels were also significant lower in the RIPC group (p<0.01). The cytokines assessment showed that IL-6 levels were increased in the RIPC group after 1h of reperfusion, in comparison to the I/R group (p<0.05). Interleukin-10 levels in RIPC groups did not differ significantly from I/R group.

CONCLUSIONS:

Remote ischemic preconditioning is effective in decreasing liver necrosis in a rat model of ischemia-reperfusion. The IL-6 expression is up-regulated and peaked at 60 min of reperfusion. There was no difference in IL-10 expression between the groups. .
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Ischemic Preconditioning / Disease Models, Animal / Liver Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Rio de Janeiro/BR

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Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Ischemic Preconditioning / Disease Models, Animal / Liver Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Rio de Janeiro/BR