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Expression analysis of notsh signaling pathway molecules in shed cultured in keratinocyte growth medium
Taha, Siti Aisyah Mohd; Ling, Joanne Koh Su; Azmi, Nur Izyan Binti; Kannan, Thirumulu Ponnuraj; Azlina, Ahmad; Mokhtar, Khairani Idah.
  • Taha, Siti Aisyah Mohd; Universiti Sains Malaysia. School of Dental Sciences. Kota Bharu. MY
  • Ling, Joanne Koh Su; Universiti Sains Malaysia. School of Dental Sciences. Kota Bharu. MY
  • Azmi, Nur Izyan Binti; Universiti Sains Malaysia. School of Dental Sciences. Kota Bharu. MY
  • Kannan, Thirumulu Ponnuraj; Universiti Sains Malaysia. School of Dental Sciences. Kota Bharu. MY
  • Azlina, Ahmad; Universiti Sains Malaysia. School of Dental Sciences. Kota Bharu. MY
  • Mokhtar, Khairani Idah; International Islamic Universiti Malaysia. Kulliyah of Dentistry. Kuantan. MY
Braz. j. oral sci ; 14(2): 135-140, Apr.-June 2015. tab, ilus
Article in English | LILACS | ID: lil-755036
ABSTRACT

Aim:

To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).

Methods:

RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green.

Results:

Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7.

Conclusions:

Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Stem Cells / Tooth, Deciduous / Gene Expression / Keratinocytes / Culture Media / Receptors, Notch Limits: Female / Humans / Male Language: English Journal: Braz. j. oral sci Journal subject: Dentistry Year: 2015 Type: Article / Project document Affiliation country: Malaysia Institution/Affiliation country: International Islamic Universiti Malaysia/MY / Universiti Sains Malaysia/MY

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Full text: Available Index: LILACS (Americas) Main subject: Stem Cells / Tooth, Deciduous / Gene Expression / Keratinocytes / Culture Media / Receptors, Notch Limits: Female / Humans / Male Language: English Journal: Braz. j. oral sci Journal subject: Dentistry Year: 2015 Type: Article / Project document Affiliation country: Malaysia Institution/Affiliation country: International Islamic Universiti Malaysia/MY / Universiti Sains Malaysia/MY