Expression analysis of notsh signaling pathway molecules in shed cultured in keratinocyte growth medium
Braz. j. oral sci
;
14(2): 135-140, Apr.-June 2015. tab, ilus
Article
in English
| LILACS
| ID: lil-755036
ABSTRACT
Aim:
To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).Methods:
RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green.Results:
Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7.Conclusions:
Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Stem Cells
/
Tooth, Deciduous
/
Gene Expression
/
Keratinocytes
/
Culture Media
/
Receptors, Notch
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2015
Type:
Article
/
Project document
Affiliation country:
Malaysia
Institution/Affiliation country:
International Islamic Universiti Malaysia/MY
/
Universiti Sains Malaysia/MY
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