Th2 cells and the ifn-γ R1 subunit in early and advanced experimental periodontitis in rats; an immunohistochemical study
Braz. j. oral sci
;
14(2): 106-111, Apr.-June 2015. ilus
Article
in English
| LILACS
| ID: lil-755038
ABSTRACT
Aim: To evaluate the involvement of Th2 cells in different periods of the active phase of experimental periodontal disease and expression of the R1 subunit of the receptor for IFN-γ during the early and advanced progression of the disease. Methods: Experimental periodontitis was induced in 30 male Wistar rats by placing cotton ligatures around the mandibular first molars. The rats were then randomly assigned into two groups: G1=15 and G2=15, in group G1, ligatures were maintained for 2 days, a period that corresponds to the initial stage of periodontal disease in rats, in G2 ligatures were left for 15 days, a period that corresponds to the advanced stage of periodontal disease. The contra-lateral teeth served as controls (unligated). An immunohistochemical investigation of the gingival tissue was performed to detect the presence of the Th2 specific transcription factor (GATA3). Results Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. IFN-γ R1 was detected at an early stage during the active phase of disease, but the expression of positive cells remained unaltered during the remaining period of the study.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Periodontal Diseases
/
Periodontitis
/
Immunohistochemistry
/
Cytokines
/
Th1 Cells
Limits:
Animals
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Potiguar/BR
Similar
MEDLINE
...
LILACS
LIS
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Periodontal Diseases
/
Periodontitis
/
Immunohistochemistry
/
Cytokines
/
Th1 Cells
Limits:
Animals
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Potiguar/BR