An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Baysediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Adaptation, Physiological
/
Pseudoalteromonas
/
Agar
/
Glycoside Hydrolases
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2015
Type:
Article
/
Project document
Affiliation country:
Switzerland
Institution/Affiliation country:
The First Institute of Oceanography/CH
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Adaptation, Physiological
/
Pseudoalteromonas
/
Agar
/
Glycoside Hydrolases
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2015
Type:
Article
/
Project document
Affiliation country:
Switzerland
Institution/Affiliation country:
The First Institute of Oceanography/CH
Consulta Detalhada
(instance:"regional") AND ( year_cluster:("2002") AND pais_afiliacao:("^iUnited States^eEstados"))(instance:"regional") AND ( year_cluster:("2002") AND pais_afiliacao:("^iUnited States^eEstados"))(instance:"regional") AND ( year_cluster:("2002") AND pais_afiliacao:("^iUnited States^eEstados"))(instance:"regional") AND ( year_cluster:("2002") AND pais_afiliacao:("^iUnited States^eEstados"))