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Molecular profiling of drug resistant isolates ofMycobacterium tuberculosis in the state of Santa Catarina, southern Brazil
Prim, Rodrigo Ivan; Schörner, Marcos André; Senna, Simone Gonçalves; Nogueira, Christiane Lourenço; Figueiredo, Anna Carolina Cançado; Oliveira, Jaquelline Germano de; Rovaris, Darcita Bürger; Bazzo, Maria Luiza.
  • Prim, Rodrigo Ivan; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
  • Schörner, Marcos André; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
  • Senna, Simone Gonçalves; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
  • Nogueira, Christiane Lourenço; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
  • Figueiredo, Anna Carolina Cançado; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
  • Oliveira, Jaquelline Germano de; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
  • Rovaris, Darcita Bürger; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
  • Bazzo, Maria Luiza; Universidade Federal de Santa Catarina. Laboratório de Biologia Molecular, Sorologia e Micobactérias. Florianópolis. BR
Mem. Inst. Oswaldo Cruz ; 110(5): 618-623, Aug. 2015. tab, ilus
Article in English | LILACS | ID: lil-755891
ABSTRACT

Drug resistance is a global threat and one of the main contributing factors to tuberculosis (TB) outbreaks. The goal of this study was to analyse the molecular profile of multidrug-resistant TB (MDR-TB) in the state of Santa Catarina in southern Brazil. Fifty-three MDR Mycobacterium tuberculosisclinical isolates were analysed by spoligotyping and a partial region of therpoB gene, which is associated with rifampicin resistance (RMP-R), was sequenced. Some isolates were also distinguished by their mycobacterial interspersed repetitive units (MIRU). S531L was the most prevalent mutation found within rpoBin RMP-R isolates (58.5%), followed by S531W (20.8%). Only two MDR isolates showed no mutations withinrpoB. Isolates of the Latin American Mediterranean (LAM) family were the most prevalent (45.3%) found by spoligotyping, followed by Haarlem (9.4%) and T (7.5%) families. SIT106 was found in 26.4% of isolates and all SIT106 isolates typed by MIRU-12 (5 out of 14) belong to MIT251. There was a high correlation between the S531W mutation and the LAM family mainly because all SIT2263 (LAM9) isolates carry this mutation. Among isolates with the S531W mutation in rpoB MIRU demonstrates a cluster formed by four isolates (SIT2263 and MIT163) and very similar profiles were observed between eight of the nine isolates. Better characterisation of TB isolates may lead to new ways in which to control and treat TB in this region of Brazil.

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Full text: Available Index: LILACS (Americas) Main subject: DNA, Bacterial / Tuberculosis, Multidrug-Resistant / Drug Resistance, Multiple, Bacterial / Mutation / Mycobacterium tuberculosis / Antitubercular Agents Limits: Adult / Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Catarina/BR

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Full text: Available Index: LILACS (Americas) Main subject: DNA, Bacterial / Tuberculosis, Multidrug-Resistant / Drug Resistance, Multiple, Bacterial / Mutation / Mycobacterium tuberculosis / Antitubercular Agents Limits: Adult / Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Catarina/BR