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A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain
Weiss, M; Brum, MCS; Anziliero, D; Weiblen, R; Flores, EF.
  • Weiss, M; Universidade Federal de Santa Maria. Departamento de Medicina Veterinária Preventiva. Setor de Virologia. Santa Maria. BR
  • Brum, MCS; Universidade Federal de Santa Maria. Departamento de Medicina Veterinária Preventiva. Setor de Virologia. Santa Maria. BR
  • Anziliero, D; Universidade Federal de Santa Maria. Departamento de Medicina Veterinária Preventiva. Setor de Virologia. Santa Maria. BR
  • Weiblen, R; Universidade Federal de Santa Maria. Departamento de Medicina Veterinária Preventiva. Setor de Virologia. Santa Maria. BR
  • Flores, EF; Universidade Federal de Santa Maria. Departamento de Medicina Veterinária Preventiva. Setor de Virologia. Santa Maria. BR
Braz. j. med. biol. res ; 48(9): 843-851, Sept. 2015. tab, ilus
Article in English | LILACS | ID: lil-756410
ABSTRACT
A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects on replication efficacy in vitro. Thirteen calves inoculated intramuscularly with BoHV-1△gE developed virus neutralizing antibodies at day 42 post-infection (titers from 2 to 16), demonstrating the ability of the recombinant to replicate and to induce a serological response in vivo. Furthermore, the serological response induced by recombinant BoHV-1△gE could be differentiated from that induced by wild-type BoHV-1 by the use of an anti-gE antibody ELISA kit. Taken together, these results indicated the potential application of recombinant BoHV-1 △gE in vaccine formulations to prevent the losses caused by BoHV-1 infections while allowing for differentiation of vaccinated from naturally infected animals.
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Full text: Available Index: LILACS (Americas) Main subject: Viral Proteins / Viral Vaccines / Gene Deletion / Herpesvirus 1, Bovine Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Maria/BR

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Full text: Available Index: LILACS (Americas) Main subject: Viral Proteins / Viral Vaccines / Gene Deletion / Herpesvirus 1, Bovine Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Santa Maria/BR