Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+
Braz. j. med. biol. res
;
48(10): 902-907, Oct. 2015. tab, ilus
Article
in English
| LILACS
| ID: lil-761597
ABSTRACT
Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcoholchloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI)6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanolchloroform (2575), Whatman 3MM paper and ethanolchloroform (2575), and the more expensive ITLC-SG and 1-propanolchloroform (1090) were suitable systems for the direct determination of radiochemical purity of [99mTc(MIBI)6]+ since impurities such as99mTc-reduced-hydrolyzed (RH),99mTcO4- and [99mTc(cysteine)2]-complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Chromatography, Paper
/
Chromatography, Thin Layer
/
Radiopharmaceuticals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto do Câncer do Estado de São Paulo/BR
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