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Activation of endogenous angiotensin converting enzyme 2 prevents early injuries induced by hyperglycemia in rat retina
Foureaux, G.; Nogueira, B. S.; Coutinho, D. C. O.; Raizada, M. K.; Nogueira, J. C.; Ferreira, A. J..
  • Foureaux, G.; Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte. BR
  • Nogueira, B. S.; Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte. BR
  • Coutinho, D. C. O.; Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte. BR
  • Raizada, M. K.; Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte. BR
  • Nogueira, J. C.; Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte. BR
  • Ferreira, A. J.; Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte. BR
Braz. j. med. biol. res ; 48(12): 1109-1114, Dec. 2015. graf
Article in English | LILACS | ID: lil-762913
ABSTRACT
Diabetic retinopathy (DR) is a serious complication of diabetes mellitus that may result in blindness. We evaluated the effects of activation of endogenous angiotensin converting enzyme (ACE) 2 on the early stages of DR. Rats were administered an intravenous injection of streptozotocin to induce hyperglycemia. The ACE2 activator 1-[[2-(dimethylamino) ethyl] amino]-4-(hydroxymethyl)-7-[[(4-methylphenyl) sulfonyl] oxy]-9H-xanthone 9 (XNT) was administered by daily gavage. The death of retinal ganglion cells (RGC) was evaluated in histological sections, and retinal ACE2, caspase-3, and vascular endothelial growth factor (VEGF) expressions were analyzed by immunohistochemistry. XNT treatment increased ACE2 expression in retinas of hyperglycemic (HG) rats (control 13.81±2.71 area%; HG 14.29±4.30 area%; HG+XNT 26.87±1.86 area%; P<0.05). Importantly, ACE2 activation significantly increased the RCG number in comparison with HG animals (control 553.5±14.29; HG 530.8±10.3 cells; HG+XNT 575.3±16.5 cells; P<0.05). This effect was accompanied by a reduction in the expression of caspase-3 in RGC of the HG+XNT group when compared with untreated HG rats (control 18.74±1.59; HG 38.39±3.39 area%; HG+XNT 27.83±2.80 area%; P<0.05). Treatment with XNT did not alter the VEGF expression in HG animals (P>0.05). Altogether, these findings indicate that activation of ACE2 reduced the death of retinal ganglion cells by apoptosis in HG rats.
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Full text: Available Index: LILACS (Americas) Main subject: Retinal Diseases / Peptidyl-Dipeptidase A / Secondary Prevention / Hyperglycemia Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR

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Full text: Available Index: LILACS (Americas) Main subject: Retinal Diseases / Peptidyl-Dipeptidase A / Secondary Prevention / Hyperglycemia Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Minas Gerais/BR