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POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells
França, M. M.; Abreu, N. P.; Vrechi, T. A. M.; Lotfi, C. F..
  • França, M. M.; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Anatomia. São Paulo. BR
  • Abreu, N. P.; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Anatomia. São Paulo. BR
  • Vrechi, T. A. M.; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Anatomia. São Paulo. BR
  • Lotfi, C. F.; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Anatomia. São Paulo. BR
Braz. j. med. biol. res ; 48(12): 1087-1094, Dec. 2015. graf
Article in English | LILACS | ID: lil-762914
ABSTRACT
During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1 overexpression, following the transfection of pCMVMycPod-1, significantly decreased the endogenous levels of Sf-1 mRNA and protein in F/R cells, but not in G cells, and also decreased the expression of the SF-1 target StAR in F/R cells. In G cells overexpressing POD-1, no modulation of the expression of SF-1 targets, StAR and CYP11B2, was observed. Our data showing that G and F/R cells respond differently to ectopic POD-1 expression emphasize the functional differences between the outer and inner zones of the adrenal cortex, and support the hypothesis that SF-1 is regulated by POD-1/Tcf21 in normal adrenocortical cells lacking the alterations in cellular physiology found in tumor cells.
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Full text: Available Index: LILACS (Americas) Main subject: Phosphoproteins / Adrenal Cortex / Basic Helix-Loop-Helix Transcription Factors / Steroidogenic Factor 1 Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Phosphoproteins / Adrenal Cortex / Basic Helix-Loop-Helix Transcription Factors / Steroidogenic Factor 1 Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR